Assessing spread of phytophthoras in Scottish forests by recreational and harvesting activities using comparative qPCR and metabarcoding techniques
Impact: Add to the evidence base underpinning public and sector-facing biosecurity campaigns and improve disease management and restrict spread of the disease.
Assessing spread of Phytophthoras in Scottish forests by recreational and harvesting activities using comparative qPCR and metabarcoding techniques
Pathogens from the genus Phytophthora pose some of the greatest threats to tree health and are likely to become increasingly prevalent in the UK as the climate becomes warmer and wetter. This project added new data based on a DNA metabarcoding approach to determine the Phytophthora species diversity in soil samples and sampled material from timber harvesting operations alongside those of recreational activities.
Metabarcoding analysis of Phytophthora diversity in spore traps and implications for disease forecasting in the P. ramorum management zone
Surveillance and monitoring of airborne pathogens is a key tool in the management of healthy forests and controlling disease outbreaks. A Scottish Forestry-funded project was carried out in autumn 2019 to validate different spore-trapping techniques for monitoring airborne P. ramorum inoculum using a species-specific qPCR assay. This project aimed to add further value to the Scottish Forestry project by investigating the suitability of DNA metabarcoding for screening spore trap samples for P. ramorum and other Phytophthora species, seeking early data for aerially dispersed Phytophthora species that may become problematic in UK forests. The study highlighted the value of both monitoring P. ramorum dispersal and detecting other Phytophthora species, to predict and understand changes in disease severity in UK tree host species. Recommendations included that the use of both rain and wind-borne inoculum capture methods might be advantageous due to the variety of weather conditions under which inoculum can disperse. Lineage testing in Southwest Scotland should be carried out to look at the prevalence of lineage EU1 in the EU2 area and to monitor for presence of North American lineages. Development of a new DNA barcode more specific to Phytophthora would be beneficial.
Status, Scottish specific issues, Plant Health Centre perspective and Key priorities and recommendations concerning the threat from Phytophthora ramorum to Scotland